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	Provedor de dados Anais da ABC (AABC) (5) BJPS (1) Autor Sola-Penna,Mauro (6) Alves,Gutemberg G. (2) Alviano,Celuta S. (1) Araújo,Alexandre H.B. (1) Atella,Georgia C. (1) Barbosa,Gleyce M. (1) Girard-Dias,Wendell (1) Grazinoli-Garrido,Rodrigo (1) Guerreiro,Luiz H. (1) Guerreiro,Luiz Henrique (1) Holandino,Carla (1) Lima,Luís M.T.R. (1) Lima,Luís Mauricio Trambaioli da Rocha e (1) Marinho-Carvalho,Monica M. (1) Mascarenhas,Camile Moreira (1) Menezes,Fábio S. (1) Miranda,Kildare (1) Mizurini,Daniella M. (1) Rennó,Magdalena N. (1) Ricci Júnior,Eduardo (1) Mais... Palavra-chave Metabolism (2) Phosphofructokinase (2) Actin (1) Alcohol (1) Amylin (1) Antitumoral agents (1) Diabetes (1) Enzyme (1) Ethanol extract (1) Glycemia (1) Glycolysis (1) Hormone (1) Human insulin/study (1) Hypoglycemia (1) Insect (1) Islet associated polypeptide (1) Mice (1) Microparticles (1) Orbignya speciosa (1) Organic solvents (1) Mais... Tipo do documento Info:eu-repo/semantics/article (6) Ano 2017 (1) 2013 (1) 2008 (1) 2007 (1) 2004 (2) País Brazil (6) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus Anais da ABC (AABC) Alves,Gutemberg G.; Marinho-Carvalho,Monica M.; Atella,Georgia C.; Silva-Neto,Mario A.C.; Sola-Penna,Mauro. 6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphofructokinase; Metabolism; Insect. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008 Amylin induces hypoglycemia in mice Anais da ABC (AABC) Guerreiro,Luiz H.; Silva,Daniel DA; Sola-Penna,Mauro; Mizurini,Daniella M.; Lima,Luís M.T.R.. Amylin is a 37-aminoacid pancreatic protein that exerts control over several metabolic events such as glycemia and lacticemia. Amylin has long been shown to induce increases in arterial plasma glucose. We decided to investigate whether amylin plays additional roles in the glucose metabolism. We evaluated glucose homeostasis using whole blood from the tail tip of fasting, conscious, unrestrained normal and streptozotocyn-induced diabetic mice following subcutaneous administration of mouse amylin. Subcutaneous injection of 1 μg mouse amylin caused a transient decrease in whole blood glucose in both normal and diabetic mice in the absence of insulin. The blood glucose levels were lowest approximately 2 hours after amylin administration, after that they... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylin; Islet associated polypeptide; Glycemia; Diabetes; Mice; Hypoglycemia. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100349 Crude ethanol extract from babassu (Orbignya speciosa): cytotoxicity on tumoral and non-tumoral cell lines Anais da ABC (AABC) Rennó,Magdalena N.; Barbosa,Gleyce M.; Zancan,Patricia; Veiga,Venicio F.; Alviano,Celuta S.; Sola-Penna,Mauro; Menezes,Fábio S.; Holandino,Carla. Plant-derived substances have been considered as important sources of drugs, including antineoplasic agents. Babassu mesocarp is popularly used in Brazil as a food additive, and in popular medicine against several conditions, such as inflammations, menstrual pains and leukaemia. From babassu Orbignya speciosa (Mart.) Barb. Rodr. [Arecaceae (Palmae)] epicarp/mesocarp, an ethanol extract was prepared and named OSEME, which was tested on the viability,morphology and metabolism of several cell lines, such as the leukaemic cell lines, HL-60, K562 and the latter multidrug resistant counterpart K562-Lucena 1, the human breast cancer cell line MCF-7, the mouse fibroblast cell line 3T3-L1 and fresh human lymphocytes. OSEME promoted a dose-dependent decrease on the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Antitumoral agents; Ethanol extract; Orbignya speciosa. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000300008 Effects of insulin and actin on phosphofructokinase activity and cellular distribution in skeletal muscle Anais da ABC (AABC) Silva,Ana Paula P.; Alves,Gutemberg G.; Araújo,Alexandre H.B.; Sola-Penna,Mauro. In this work, we report evidences that the association of phosphofructokinase and F-actin can be affected by insulin stimulation in rabbit skeletal muscle homogenates and that this association can be a mechanism of phos-phofructokinase regulation. Through co-sedimentation techniques, we observed that on insulin-stimulated tissues, approximately 70% of phosphofructokinase activity is co-located in an actin-enriched fraction, against 28% in control. This phenomenon is accompanied by a 100% increase in specific phosphofructokinase activity in stimulated homogenates. Purified F-actin causes an increase of 230% in phosphofructokinase activity and alters its kinetic parameters. The presence of F-actin increases the affinity of phosphofructokinase for fructose... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphofructokinase; Hormone; Rabbit; Actin; Metabolism; Glycolysis. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000300008 Inactivation of yeast inorganic pyrophosphatase by organic solvents Anais da ABC (AABC) Grazinoli-Garrido,Rodrigo; Sola-Penna,Mauro. A number of application for enzymes in organic solvents have been developed in chemical processing, food related conversions and analyses. The only unsolved problem related to nonaqueous enzymology is the notion that enzymes in organic solvent are mostly far less active than in water. Therefore, studies concerning the mechanisms by which enzymes are inactivated by organic solvents would reveal a clear understanding of the structure-function relationship of this phenomenon. Here we analyzed the effects of a series of alcohols (methanol, ethanol, 1-propanol and 2-propanol) and acetone on the activity of yeast inorganic pyrophosphatase. We observed that solvents inactivated the enzyme in a dose-dependent manner. This inactivation is also dependent on the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Organic solvents; Enzyme; Yeast inorganic pyrophosphatase; Alcohol. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400006 Macromolecular confinement of therapeutic protein in polymeric particles for controlled release: insulin as a case study BJPS Guerreiro,Luiz Henrique; Silva,Daniel da; Girard-Dias,Wendell; Mascarenhas,Camile Moreira; Miranda,Kildare; Sola-Penna,Mauro; Ricci Júnior,Eduardo; Lima,Luís Mauricio Trambaioli da Rocha e. ABSTRACT Sustained release systems for therapeutic proteins have been widely studied targeting to improve the action of these drugs. Molecular entrapping of proteins is particularly challenging due to their conformational instability. We have developed a micro-structured poly-epsilon-caprolactone (PCL) particle system loaded with human insulin using a simple double-emulsion w/o/w method followed by solvent evaporation method. This formulation is comprised by spheric-shaped microparticles with average size of 10 micrometers. In vitro release showed a biphasic behavior such as a rapid release with about 50% of drug delivered within 2 hours and a sustained phase for up to 48 h. The subcutaneous administration of microencapsulated insulin showed a biphasic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human insulin/study; Microparticles; Poly-ε-caprolactone; Therapeutic proteins/study/action; Proteins/molecular entrapping. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200622 Registros recuperados: 6 Primeira ... 1 ... Última

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